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Cannabis has drawn attention due to its possible medical benefits, including antibacterial activity along with its other useful properties. In this study, two pathogens were used namely Candida albicans (Fungi) and Staphylococcus aureus (Bacteria)to assess the antimicrobial activity of cannabis. In other research, cannabis has shown that the presence of the naturally occurring compounds can prevent the growth of certain microorganisms and can eventually help to cure diseases caused by such microorganisms. There are two main methods used in this study the Agar Well Diffusion Method and the Bacterial Growth Kinetics Test (OD600). Bacterial Growth Kinetics Test plate assay test is also performed, all methods were performed to see the difference of growth of both microorganisms between the Concentrations that are made (100, 200, 300, 400, Pure Extract), also 5% DMSO is also tested along with the positive and negative Controls. The hypothesis is proven, as cannabis displayed antimicrobial activity against C. albicans and S. aureus, and both are dose dependent. Although, for C. albicans a higher dosage might be able to show visible effectivity. However, future research is needed to clarify the mechanisms of action, improve formulations, assess safety profiles due to inconsistencies on certain results and further study about the clinical evidence. By understanding the antimicrobial properties of cannabis in relation to the two microorganisms being studied in this research it may help in the discovery and creation of innovative therapeutic approaches for clinical diseases.
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