SBP 21 - Antimicrobial kitchen wipes embedded with silver nanoparticles

This project explores the use of silver nanoparticles (AgNPs) to enhance the antimicrobial properties of cotton wipes. Silver nanoparticles are known for their unique properties, including high surface area and potent antimicrobial effects. The project focuses on synthesizing and applying two concentrations of AgNPs (0.001 M and 0.003 M) to cotton wipes to assess their effectiveness in inhibiting microbial growth. The study investigates the antimicrobial potential of AgNPs coated cotton wipes against four different microorganisms: E. coli, S. aureus, S. enteritidis and C. albicans. The aim is to develop a sustainable alternative to conventional antimicrobial wipes, which often involve the use of harsh chemicals that may be harmful to the environment.

The core function of this project was to test and enhance the antimicrobial properties of cotton wipes using silver nanoparticles. AgNPs, when applied to cotton wipes, are expected to impart antimicrobial cell membranes and interfere with essential cellular processes. The project synthesized AgNPs at two different concentrations (0.001 M and 0.003 M), characterized using UV-Visible spectrophotometry, and evaluated their effectiveness in killing or inhibiting the growth of various pathogens.

The sterile cotton swatches were soaked in both of the AgNPs solutions overnight. After that, they were dried and reserved.

The effectiveness of AgNPs coated wipes is tested by duplicates through several experimental methods. First, well diffusion tests were used to determine the antimicrobial activity of AgNPs against the selected microorganisms. Second, the broth method test examined the antimicrobial properties of AgNPs coated wipes by evaluating their ability to reduce microbial growth on the fabric. Finally, the results from these tests were compared to assess the optimal concentration of AgNPs for maximum efficacy.

The antimicrobial properties of the AgNPs coated wipes are particularly significant for bacterial pathogens like E. coli, S. aureus, and S. enteritidis, with the wipes showing a marked reduction in microbial load. However, the antimicrobial effect against Candida albicans was not as pronounced, which suggests further optimization of the experimental conditions may be needed to improve efficacy against fungal pathogens.

This project was created to address antimicrobial resistance (AMR) by exploring silver nanoparticles (AgNPs) as a sustainable alternative to conventional antimicrobial agents. AgNPs have proven antimicrobial properties and can target a wide range of pathogens. By incorporating AgNPs into cotton wipes, the project aims to create an environmentally friendly, effective solution for reducing microbial contamination, while replacing harmful chemicals in traditional disinfecting wipes. This approach not only addresses the growing issue of antimicrobial resistance but also seeks to reduce the environmental impact of single-use wipes, promoting sustainability and waste reduction.